Reversal of Reserpine-induced Orofacial Dyskinesia by Chlorogenic Acid in Rats

Background and Objectives: Chlorogenic acid, a natural polyphenolic compound has been reported to possess neuroprotective and antioxidant activities, but the effect of chlorogenic acid on orofacial dyskinesia has not been explored till date, so the aim of the present study was to investigate the effect of chlorogenic acid on reserpine-induced orofacial dyskinesia in Wistar male albino rats. Methodology: Reserpine (1 mg kgG1) was injected by intraperitoneal route for 3 alternate days (day 1, 3 and 5) to induce orofacial dyskinesia in rats. Chlorogenic acid (10, 20 and 40 mg kgG1, p.o.) was administered from 6-20th day (for 15 successive days) to separate groups of rats pretreated with reserpine. Results: Administration of reserpine for 3 alternate days significantly increased vacuous chewing movements and also caused hypolocomotion in rats. Treatment with chlorogenic acid (10, 20 and 40 mg kgG1) significantly ameliorated reserpine-induced vacuous chewing movements and hypolocomotion. Reserpine significantly increased lipid peroxidation and decreased the levels of reduced glutathione and activities of catalase and superoxide dismutase in rat brain. Chlorogenic acid significantly reversed reserpine-induced oxidative stress, as indicated by decrease in lipid peroxidation and increase in reduced glutathione levels, catalase and superoxide dismutase activities. Conclusion: Chlorogenic acid showed significant protective effect against reserpine-induced orofacial dyskinesia, probably through amelioration of oxidative stress in rat brain.